Phosphorus in PDB 1ajk: Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84

All present enzymatic activity of Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84:
3.2.1.73;

The structure of Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84, PDB code: 1ajk was solved by J.Ay, U.Heinemann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.33 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.450, 65.820, 69.610, 90.00, 106.87, 90.00
R / Rfree (%)	16.8 / 21.9

The binding sites of Phosphorus atom in the Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84 (pdb code 1ajk). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total only one binding site of Phosphorus was determined in the Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84, PDB code: 1ajk:

Phosphorus binding site 1 out of 1 in the Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase CPA16M-84 within 5.0Å range: probe atom residue distance (Å) B Occ A:P308 b:40.3 occ:1.00 P A:PO4308 0.0 40.3 1.0 O1 A:PO4308 1.5 44.6 1.0 O3 A:PO4308 1.5 45.2 1.0 O2 A:PO4308 1.5 47.1 1.0 O4 A:PO4308 1.6 49.1 1.0 N A:ASP56 3.6 17.2 1.0 CA A:PHE55 4.2 14.6 1.0 CB A:ASP56 4.2 15.4 1.0 C A:PHE55 4.3 15.0 1.0 CB A:PHE55 4.3 17.8 1.0 CA A:ASP56 4.5 15.2 1.0 O A:ASP56 5.0 13.4 1.0

J.Ay, M.Hahn, K.Decanniere, K.Piotukh, R.Borriss, U.Heinemann. Crystal Structures and Properties of De Novo Circularly Permuted 1,3-1,4-Beta-Glucanases. Proteins V. 30 155 1998.
ISSN: ISSN 0887-3585
PubMed: 9489923
DOI: 10.1002/(SICI)1097-0134(19980201)30:2<155::AID-PROT5>3.0.CO;2-M