Phosphorus in PDB 1ahi: 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid

Enzymatic activity of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid

All present enzymatic activity of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid:
1.1.1.159;

Protein crystallography data

The structure of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid, PDB code: 1ahi was solved by N.Tanaka, T.Nonaka, Y.Mitsui, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 2.30
Space group P 41 21 2
Cell size a, b, c (Å), α, β, γ (°) 81.590, 81.590, 214.650, 90.00, 90.00, 90.00
R / Rfree (%) 18 / 25

Phosphorus Binding Sites:

The binding sites of Phosphorus atom in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid (pdb code 1ahi). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 4 binding sites of Phosphorus where determined in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid, PDB code: 1ahi:
Jump to Phosphorus binding site number: 1; 2; 3; 4;

Phosphorus binding site 1 out of 4 in 1ahi

Go back to Phosphorus Binding Sites List in 1ahi
Phosphorus binding site 1 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P302

b:69.8
occ:1.00
 PA A:NAI302 0.0 69.8 1.0
O2A A:NAI302 1.5 67.0 1.0
O1A A:NAI302 1.5 72.5 1.0
O3 A:NAI302 1.6 70.2 1.0
O5B A:NAI302 1.6 67.2 1.0
C5B A:NAI302 2.6 61.6 1.0
PN A:NAI302 3.0 72.2 1.0
O2N A:NAI302 3.4 69.2 1.0
O1N A:NAI302 3.6 72.0 1.0
C4B A:NAI302 3.9 57.3 1.0
CB A:ALA196 4.0 56.9 1.0
CG2 A:THR194 4.1 56.8 1.0
O5D A:NAI302 4.2 70.7 1.0
C3B A:NAI302 4.2 53.0 1.0
C5D A:NAI302 4.6 65.6 1.0
O A:ALA21 4.8 29.5 1.0
O4B A:NAI302 4.8 56.3 1.0
N A:ALA196 4.9 60.2 1.0
CA A:ALA196 5.0 58.8 1.0

Phosphorus binding site 2 out of 4 in 1ahi

Go back to Phosphorus Binding Sites List in 1ahi
Phosphorus binding site 2 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P302

b:72.2
occ:1.00
 PN A:NAI302 0.0 72.2 1.0
O2N A:NAI302 1.5 69.2 1.0
O1N A:NAI302 1.5 72.0 1.0
O3 A:NAI302 1.6 70.2 1.0
O5D A:NAI302 1.6 70.7 1.0
C5D A:NAI302 2.7 65.6 1.0
PA A:NAI302 3.0 69.8 1.0
O2A A:NAI302 3.3 67.0 1.0
O5B A:NAI302 3.6 67.2 1.0
CG2 A:THR194 3.9 56.8 1.0
C4D A:NAI302 4.1 61.9 1.0
O1A A:NAI302 4.1 72.5 1.0
C5B A:NAI302 4.1 61.6 1.0
O4D A:NAI302 4.2 60.3 1.0
N7N A:NAI302 4.3 49.8 1.0
N A:ILE23 4.3 34.6 1.0
OG1 A:THR194 4.4 55.5 1.0
CB A:ALA196 4.4 56.9 1.0
C2N A:NAI302 4.5 57.1 1.0
CG1 A:ILE23 4.5 26.1 1.0
CB A:ILE23 4.5 27.5 1.0
CB A:THR194 4.5 55.4 1.0
CD1 A:ILE23 4.6 26.1 1.0
CA A:GLY22 4.7 33.4 1.0
O A:ALA21 4.9 29.5 1.0
C3D A:NAI302 5.0 62.0 1.0
C A:GLY22 5.0 31.5 1.0

Phosphorus binding site 3 out of 4 in 1ahi

Go back to Phosphorus Binding Sites List in 1ahi
Phosphorus binding site 3 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 3 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid within 5.0Å range:
probe atom residue distance (Å) B Occ
B:P302

b:48.5
occ:1.00
 PA B:NAI302 0.0 48.5 1.0
O2A B:NAI302 1.5 42.6 1.0
O1A B:NAI302 1.5 42.7 1.0
O3 B:NAI302 1.6 49.2 1.0
O5B B:NAI302 1.6 46.4 1.0
C5B B:NAI302 2.6 44.7 1.0
PN B:NAI302 3.0 53.2 1.0
O2N B:NAI302 3.5 52.8 1.0
CG2 B:THR194 3.7 47.3 1.0
O1N B:NAI302 3.8 55.8 1.0
C4B B:NAI302 3.9 43.3 1.0
O5D B:NAI302 3.9 54.7 1.0
CB B:ALA196 4.1 53.0 1.0
O B:HOH436 4.2 31.5 1.0
O B:HOH405 4.2 24.9 1.0
C3B B:NAI302 4.3 39.8 1.0
N B:ALA196 4.5 58.4 1.0
CA B:ALA196 4.8 56.2 1.0
CA B:GLY22 4.8 26.7 1.0
C5D B:NAI302 4.9 53.8 1.0
N B:GLY22 4.9 25.2 1.0
CB B:ALA21 4.9 17.6 1.0
O4B B:NAI302 5.0 42.1 1.0

Phosphorus binding site 4 out of 4 in 1ahi

Go back to Phosphorus Binding Sites List in 1ahi
Phosphorus binding site 4 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 4 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nadh and 7-Oxo Glycochenodeoxycholic Acid within 5.0Å range:
probe atom residue distance (Å) B Occ
B:P302

b:53.2
occ:1.00
 PN B:NAI302 0.0 53.2 1.0
O1N B:NAI302 1.5 55.8 1.0
O2N B:NAI302 1.5 52.8 1.0
O3 B:NAI302 1.6 49.2 1.0
O5D B:NAI302 1.6 54.7 1.0
C5D B:NAI302 2.8 53.8 1.0
PA B:NAI302 3.0 48.5 1.0
O2A B:NAI302 3.5 42.6 1.0
O5B B:NAI302 3.5 46.4 1.0
CG2 B:THR194 3.6 47.3 1.0
O B:HOH405 3.6 24.9 1.0
O4D B:NAI302 3.9 54.0 1.0
C4D B:NAI302 3.9 57.0 1.0
O1A B:NAI302 4.1 42.7 1.0
C5B B:NAI302 4.1 44.7 1.0
C2N B:NAI302 4.1 61.1 1.0
N7N B:NAI302 4.2 73.4 1.0
N B:ILE23 4.3 24.6 1.0
CD1 B:ILE23 4.4 11.6 1.0
CB B:ALA196 4.5 53.0 1.0
CG1 B:ILE23 4.5 13.6 1.0
CB B:THR194 4.5 52.0 1.0
CB B:ILE23 4.6 12.6 1.0
OG1 B:THR194 4.6 49.2 1.0
C1D B:NAI302 4.8 60.7 1.0
CA B:GLY22 4.8 26.7 1.0
C3D B:NAI302 4.9 58.6 1.0
C2D B:NAI302 4.9 60.4 1.0
N1N B:NAI302 5.0 62.1 1.0

Reference:

N.Tanaka, T.Nonaka, T.Tanabe, T.Yoshimoto, D.Tsuru, Y.Mitsui. Crystal Structures of the Binary and Ternary Complexes of 7 Alpha-Hydroxysteroid Dehydrogenase From Escherichia Coli. Biochemistry V. 35 7715 1996.
ISSN: ISSN 0006-2960
PubMed: 8672472
DOI: 10.1021/BI951904D
Page generated: Fri Sep 25 12:56:45 2020

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy