Phosphorus in PDB 1ahh: 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+

All present enzymatic activity of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+:
1.1.1.159;

The structure of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+, PDB code: 1ahh was solved by N.Tanaka, T.Nonaka, Y.Mitsui, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.30
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	81.660, 81.660, 214.600, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 26.5

The binding sites of Phosphorus atom in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+ (pdb code 1ahh). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 4 binding sites of Phosphorus where determined in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+, PDB code: 1ahh:
Jump to Phosphorus binding site number: 1; 2; 3; 4;

Phosphorus binding site 1 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+ within 5.0Å range: probe atom residue distance (Å) B Occ A:P256 b:63.4 occ:1.00 PA A:NAD256 0.0 63.4 1.0 O1A A:NAD256 1.5 62.8 1.0 O2A A:NAD256 1.5 64.9 1.0 O5B A:NAD256 1.6 56.6 1.0 O3 A:NAD256 1.6 64.4 1.0 C5B A:NAD256 2.6 49.6 1.0 PN A:NAD256 3.1 61.2 1.0 O2N A:NAD256 3.5 62.1 1.0 O1N A:NAD256 3.8 58.9 1.0 C4B A:NAD256 3.9 44.8 1.0 C3B A:NAD256 4.1 38.1 1.0 O5D A:NAD256 4.1 52.3 1.0 O A:HOH259 4.6 19.8 1.0 C2B A:NAD256 4.8 36.0 1.0 O4B A:NAD256 4.9 42.5 1.0 C5D A:NAD256 4.9 42.6 1.0

Phosphorus binding site 2 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+ within 5.0Å range: probe atom residue distance (Å) B Occ A:P256 b:61.2 occ:1.00 PN A:NAD256 0.0 61.2 1.0 O2N A:NAD256 1.5 62.1 1.0 O1N A:NAD256 1.5 58.9 1.0 O5D A:NAD256 1.6 52.3 1.0 O3 A:NAD256 1.6 64.4 1.0 C5D A:NAD256 2.7 42.6 1.0 PA A:NAD256 3.1 63.4 1.0 O2A A:NAD256 3.5 64.9 1.0 O5B A:NAD256 3.7 56.6 1.0 O A:HOH259 3.9 19.8 1.0 C4D A:NAD256 3.9 38.9 1.0 O4D A:NAD256 4.1 37.6 1.0 O1A A:NAD256 4.2 62.8 1.0 C2N A:NAD256 4.2 28.5 1.0 N7N A:NAD256 4.2 23.9 1.0 C5B A:NAD256 4.3 49.6 1.0 C3D A:NAD256 4.5 33.6 1.0 CG2 A:THR194 4.6 56.5 1.0 C2D A:NAD256 4.6 35.6 1.0 C1D A:NAD256 4.8 33.2 1.0 N A:ILE23 4.9 33.0 1.0 CD1 A:ILE23 5.0 19.1 1.0 N1N A:NAD256 5.0 23.9 1.0

Phosphorus binding site 3 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 3 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+ within 5.0Å range: probe atom residue distance (Å) B Occ B:P256 b:55.9 occ:1.00 PA B:NAD256 0.0 55.9 1.0 O1A B:NAD256 1.5 51.7 1.0 O2A B:NAD256 1.5 51.2 1.0 O3 B:NAD256 1.6 56.6 1.0 O5B B:NAD256 1.6 50.0 1.0 C5B B:NAD256 2.6 43.4 1.0 PN B:NAD256 3.0 51.5 1.0 O1N B:NAD256 3.5 57.5 1.0 O2N B:NAD256 3.8 51.0 1.0 C4B B:NAD256 3.8 38.3 1.0 C3B B:NAD256 3.9 37.1 1.0 O5D B:NAD256 3.9 47.6 1.0 O4B B:NAD256 4.3 36.1 1.0 C5D B:NAD256 4.4 35.1 1.0 C2B B:NAD256 4.4 34.2 1.0 O B:HOH259 4.5 20.7 1.0

Phosphorus binding site 4 out of 4 in the 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 4 of 7 Alpha-Hydroxysteroid Dehydrogenase Complexed with Nad+ within 5.0Å range: probe atom residue distance (Å) B Occ B:P256 b:51.5 occ:1.00 PN B:NAD256 0.0 51.5 1.0 O1N B:NAD256 1.5 57.5 1.0 O2N B:NAD256 1.5 51.0 1.0 O3 B:NAD256 1.6 56.6 1.0 O5D B:NAD256 1.6 47.6 1.0 C5D B:NAD256 2.6 35.1 1.0 PA B:NAD256 3.0 55.9 1.0 O2A B:NAD256 3.4 51.2 1.0 O1A B:NAD256 3.8 51.7 1.0 C4D B:NAD256 3.9 32.3 1.0 N7N B:NAD256 3.9 27.6 1.0 O5B B:NAD256 4.0 50.0 1.0 C2N B:NAD256 4.0 31.2 1.0 O4D B:NAD256 4.0 34.5 1.0 O B:HOH259 4.3 20.7 1.0 C5B B:NAD256 4.3 43.4 1.0 CD1 B:ILE23 4.6 16.9 1.0 C3D B:NAD256 4.6 27.3 1.0 C2D B:NAD256 4.6 27.3 1.0 C1D B:NAD256 4.7 34.5 1.0 N1N B:NAD256 4.8 32.1 1.0 C7N B:NAD256 4.9 28.9 1.0 C3N B:NAD256 4.9 30.2 1.0 CG1 B:ILE23 5.0 11.6 1.0

N.Tanaka, T.Nonaka, T.Tanabe, T.Yoshimoto, D.Tsuru, Y.Mitsui. Crystal Structures of the Binary and Ternary Complexes of 7 Alpha-Hydroxysteroid Dehydrogenase From Escherichia Coli. Biochemistry V. 35 7715 1996.
ISSN: ISSN 0006-2960
PubMed: 8672472
DOI: 10.1021/BI951904D