Phosphorus in PDB 1ahf: Aspartate Aminotransferase Hexamutant

Enzymatic activity of Aspartate Aminotransferase Hexamutant

All present enzymatic activity of Aspartate Aminotransferase Hexamutant:
2.6.1.1;

Protein crystallography data

The structure of Aspartate Aminotransferase Hexamutant, PDB code: 1ahf was solved by V.N.Malashkevich, J.N.Jansonius, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 2.30
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 85.920, 79.390, 88.800, 90.00, 118.99, 90.00
R / Rfree (%) n/a / n/a

Phosphorus Binding Sites:

The binding sites of Phosphorus atom in the Aspartate Aminotransferase Hexamutant (pdb code 1ahf). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 2 binding sites of Phosphorus where determined in the Aspartate Aminotransferase Hexamutant, PDB code: 1ahf:
Jump to Phosphorus binding site number: 1; 2;

Phosphorus binding site 1 out of 2 in 1ahf

Go back to Phosphorus Binding Sites List in 1ahf
Phosphorus binding site 1 out of 2 in the Aspartate Aminotransferase Hexamutant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Aspartate Aminotransferase Hexamutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P410

b:29.7
occ:1.00
P A:PLP410 0.0 29.7 1.0
O1P A:PLP410 1.5 19.9 1.0
O3P A:PLP410 1.5 27.6 1.0
O2P A:PLP410 1.6 32.3 1.0
O4P A:PLP410 1.6 26.1 1.0
C5A A:PLP410 2.5 32.1 1.0
N A:GLY108 3.5 24.9 1.0
OG A:SER257 3.6 21.7 1.0
NH1 A:ARG266 3.7 18.5 1.0
OH B:TYR70 3.7 29.4 1.0
OG A:SER255 3.7 21.5 1.0
NH2 A:ARG266 3.8 21.2 1.0
OG A:SER109 3.9 32.8 1.0
C5 A:PLP410 3.9 37.6 1.0
N A:SER109 3.9 28.7 1.0
C5 A:IOP411 4.0 58.8 1.0
C4A A:PLP410 4.2 51.6 1.0
CB A:SER257 4.2 17.4 1.0
CZ A:ARG266 4.2 21.7 1.0
CA A:GLY108 4.2 29.6 1.0
CA A:GLY107 4.3 26.4 1.0
C A:GLY107 4.3 24.8 1.0
CB A:SER255 4.5 18.6 1.0
CE1 B:TYR70 4.5 21.3 1.0
C4 A:PLP410 4.5 43.4 1.0
CB A:SER109 4.5 28.6 1.0
CZ B:TYR70 4.6 19.3 1.0
C A:GLY108 4.7 32.5 1.0
C4 A:IOP411 4.8 58.0 1.0
CA A:SER109 4.8 29.5 1.0
NZ A:LYS258 4.9 53.2 1.0
C6 A:IOP411 4.9 62.6 1.0

Phosphorus binding site 2 out of 2 in 1ahf

Go back to Phosphorus Binding Sites List in 1ahf
Phosphorus binding site 2 out of 2 in the Aspartate Aminotransferase Hexamutant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Aspartate Aminotransferase Hexamutant within 5.0Å range:
probe atom residue distance (Å) B Occ
B:P412

b:30.1
occ:1.00
P B:PLP412 0.0 30.1 1.0
O3P B:PLP412 1.5 38.2 1.0
O1P B:PLP412 1.5 29.8 1.0
O4P B:PLP412 1.6 29.7 1.0
O2P B:PLP412 1.6 28.2 1.0
C5A B:PLP412 2.6 33.8 1.0
N B:GLY108 3.4 21.6 1.0
OG B:SER257 3.6 27.8 1.0
OG B:SER255 3.8 24.3 1.0
OH A:TYR70 3.8 22.7 1.0
NH2 B:ARG266 3.9 19.6 1.0
NH1 B:ARG266 3.9 25.6 1.0
C5 B:PLP412 4.0 36.2 1.0
N B:SER109 4.0 21.4 1.0
OG B:SER109 4.1 30.4 1.0
C4A B:PLP412 4.2 53.9 1.0
CB B:SER257 4.2 23.3 1.0
CA B:GLY108 4.2 23.3 1.0
CA B:GLY107 4.3 20.4 1.0
C B:GLY107 4.3 21.4 1.0
CZ B:ARG266 4.4 23.0 1.0
CB B:SER255 4.4 20.9 1.0
C4 B:PLP412 4.5 45.3 1.0
C B:GLY108 4.6 27.1 1.0
CE1 A:TYR70 4.6 16.8 1.0
CB B:SER109 4.6 26.8 1.0
CZ A:TYR70 4.7 15.0 1.0
NZ B:LYS258 4.8 55.6 1.0
CA B:SER109 4.9 26.3 1.0

Reference:

V.N.Malashkevich, J.J.Onuffer, J.F.Kirsch, J.N.Jansonius. Alternating Arginine-Modulated Substrate Specificity in An Engineered Tyrosine Aminotransferase. Nat.Struct.Biol. V. 2 548 1995.
ISSN: ISSN 1072-8368
PubMed: 7664122
DOI: 10.1038/NSB0795-548
Page generated: Fri Sep 25 12:56:26 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy