Phosphorus in PDB 1agr: Complex of ALF4-Activated Gi-Alpha-1 with RGS4
Protein crystallography data
The structure of Complex of ALF4-Activated Gi-Alpha-1 with RGS4, PDB code: 1agr
was solved by
J.J.G.Tesmer,
S.R.Sprang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
5.00 /
2.80
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
164.000,
97.200,
110.000,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
21.6 /
29.1
|
Phosphorus Binding Sites:
The binding sites of Phosphorus atom in the Complex of ALF4-Activated Gi-Alpha-1 with RGS4
(pdb code 1agr). This binding sites where shown within
5.0 Angstroms radius around Phosphorus atom.
In total 4 binding sites of Phosphorus where determined in the
Complex of ALF4-Activated Gi-Alpha-1 with RGS4, PDB code: 1agr:
Jump to Phosphorus binding site number:
1;
2;
3;
4;
Phosphorus binding site 1 out
of 4 in 1agr
Go back to
Phosphorus Binding Sites List in 1agr
Phosphorus binding site 1 out
of 4 in the Complex of ALF4-Activated Gi-Alpha-1 with RGS4
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 1 of Complex of ALF4-Activated Gi-Alpha-1 with RGS4 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:P355
b:16.9
occ:1.00
|
PB
|
A:GDP355
|
0.0
|
16.9
|
1.0
|
O2B
|
A:GDP355
|
1.5
|
2.0
|
1.0
|
O3B
|
A:GDP355
|
1.5
|
28.5
|
1.0
|
O1B
|
A:GDP355
|
1.5
|
7.8
|
1.0
|
O3A
|
A:GDP355
|
1.7
|
11.5
|
1.0
|
PA
|
A:GDP355
|
2.8
|
19.2
|
1.0
|
O1A
|
A:GDP355
|
3.1
|
28.3
|
1.0
|
AL
|
A:ALF357
|
3.2
|
12.3
|
1.0
|
F3
|
A:ALF357
|
3.2
|
19.3
|
1.0
|
MG
|
A:MG356
|
3.2
|
14.3
|
1.0
|
O2A
|
A:GDP355
|
3.4
|
20.3
|
1.0
|
O
|
A:HOH365
|
3.4
|
11.6
|
1.0
|
F4
|
A:ALF357
|
3.6
|
8.3
|
1.0
|
F2
|
A:ALF357
|
3.6
|
2.6
|
1.0
|
N
|
A:GLU43
|
3.7
|
25.9
|
1.0
|
N
|
A:GLY45
|
3.7
|
19.4
|
1.0
|
F1
|
A:ALF357
|
3.8
|
9.2
|
1.0
|
CA
|
A:GLU43
|
3.9
|
27.7
|
1.0
|
N
|
A:SER44
|
3.9
|
23.6
|
1.0
|
N
|
A:LYS46
|
4.0
|
20.2
|
1.0
|
NZ
|
A:LYS46
|
4.0
|
15.0
|
1.0
|
NH1
|
A:ARG178
|
4.0
|
22.4
|
1.0
|
O5'
|
A:GDP355
|
4.0
|
7.9
|
1.0
|
N
|
A:SER47
|
4.1
|
15.2
|
1.0
|
O
|
A:HOH364
|
4.1
|
15.1
|
1.0
|
C
|
A:GLU43
|
4.1
|
26.6
|
1.0
|
OG
|
A:SER47
|
4.3
|
19.6
|
1.0
|
CA
|
A:GLY45
|
4.4
|
17.8
|
1.0
|
C
|
A:GLY45
|
4.4
|
21.7
|
1.0
|
CE
|
A:LYS46
|
4.5
|
19.8
|
1.0
|
CB
|
A:LYS46
|
4.5
|
13.5
|
1.0
|
OG
|
A:SER44
|
4.5
|
33.8
|
1.0
|
CB
|
A:SER47
|
4.6
|
24.8
|
1.0
|
CA
|
A:LYS46
|
4.6
|
15.0
|
1.0
|
C5'
|
A:GDP355
|
4.7
|
7.8
|
1.0
|
C
|
A:SER44
|
4.7
|
23.2
|
1.0
|
CZ
|
A:ARG178
|
4.8
|
19.8
|
1.0
|
CA
|
A:SER44
|
4.8
|
22.1
|
1.0
|
CG
|
A:LYS46
|
4.8
|
2.0
|
1.0
|
C
|
A:GLY42
|
4.9
|
21.6
|
1.0
|
C
|
A:LYS46
|
4.9
|
15.8
|
1.0
|
NH2
|
A:ARG178
|
4.9
|
11.1
|
1.0
|
CA
|
A:SER47
|
4.9
|
17.5
|
1.0
|
O
|
A:GLU43
|
5.0
|
29.2
|
1.0
|
|
Phosphorus binding site 2 out
of 4 in 1agr
Go back to
Phosphorus Binding Sites List in 1agr
Phosphorus binding site 2 out
of 4 in the Complex of ALF4-Activated Gi-Alpha-1 with RGS4
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 2 of Complex of ALF4-Activated Gi-Alpha-1 with RGS4 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:P355
b:19.2
occ:1.00
|
PA
|
A:GDP355
|
0.0
|
19.2
|
1.0
|
O2A
|
A:GDP355
|
1.5
|
20.3
|
1.0
|
O1A
|
A:GDP355
|
1.5
|
28.3
|
1.0
|
O5'
|
A:GDP355
|
1.6
|
7.9
|
1.0
|
O3A
|
A:GDP355
|
1.6
|
11.5
|
1.0
|
C5'
|
A:GDP355
|
2.6
|
7.8
|
1.0
|
PB
|
A:GDP355
|
2.8
|
16.9
|
1.0
|
O2B
|
A:GDP355
|
2.9
|
2.0
|
1.0
|
O3B
|
A:GDP355
|
3.4
|
28.5
|
1.0
|
O
|
A:HOH365
|
3.5
|
11.6
|
1.0
|
OG1
|
A:THR48
|
3.7
|
37.4
|
1.0
|
NH1
|
A:ARG178
|
3.8
|
22.4
|
1.0
|
C4'
|
A:GDP355
|
3.8
|
11.3
|
1.0
|
O1B
|
A:GDP355
|
3.9
|
7.8
|
1.0
|
CA
|
A:GLY45
|
4.0
|
17.8
|
1.0
|
C3'
|
A:GDP355
|
4.0
|
15.4
|
1.0
|
N
|
A:GLY45
|
4.1
|
19.4
|
1.0
|
C
|
A:GLY45
|
4.3
|
21.7
|
1.0
|
CB
|
A:SER47
|
4.3
|
24.8
|
1.0
|
N
|
A:SER47
|
4.3
|
15.2
|
1.0
|
C2'
|
A:GDP355
|
4.4
|
22.2
|
1.0
|
N
|
A:THR48
|
4.4
|
19.7
|
1.0
|
MG
|
A:MG356
|
4.6
|
14.3
|
1.0
|
O
|
A:GLY45
|
4.6
|
24.1
|
1.0
|
CZ
|
A:ARG178
|
4.6
|
19.8
|
1.0
|
O4'
|
A:GDP355
|
4.7
|
18.2
|
1.0
|
CA
|
A:GLU43
|
4.7
|
27.7
|
1.0
|
N
|
A:LYS46
|
4.7
|
20.2
|
1.0
|
CA
|
A:SER47
|
4.8
|
17.5
|
1.0
|
C
|
A:GLU43
|
4.8
|
26.6
|
1.0
|
OG
|
A:SER47
|
4.8
|
19.6
|
1.0
|
CB
|
A:THR48
|
4.9
|
22.1
|
1.0
|
F2
|
A:ALF357
|
4.9
|
2.6
|
1.0
|
O
|
A:GLU43
|
5.0
|
29.2
|
1.0
|
|
Phosphorus binding site 3 out
of 4 in 1agr
Go back to
Phosphorus Binding Sites List in 1agr
Phosphorus binding site 3 out
of 4 in the Complex of ALF4-Activated Gi-Alpha-1 with RGS4
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 3 of Complex of ALF4-Activated Gi-Alpha-1 with RGS4 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:P355
b:27.5
occ:1.00
|
PB
|
D:GDP355
|
0.0
|
27.5
|
1.0
|
O3B
|
D:GDP355
|
1.5
|
31.5
|
1.0
|
O2B
|
D:GDP355
|
1.5
|
17.7
|
1.0
|
O1B
|
D:GDP355
|
1.5
|
17.7
|
1.0
|
O3A
|
D:GDP355
|
1.7
|
19.4
|
1.0
|
PA
|
D:GDP355
|
2.8
|
21.4
|
1.0
|
F3
|
D:ALF357
|
3.1
|
21.1
|
1.0
|
AL
|
D:ALF357
|
3.1
|
28.8
|
1.0
|
O1A
|
D:GDP355
|
3.2
|
27.2
|
1.0
|
MG
|
D:MG356
|
3.2
|
15.2
|
1.0
|
O2A
|
D:GDP355
|
3.4
|
30.6
|
1.0
|
O
|
D:HOH366
|
3.5
|
14.5
|
1.0
|
F2
|
D:ALF357
|
3.6
|
14.2
|
1.0
|
F4
|
D:ALF357
|
3.6
|
18.6
|
1.0
|
N
|
D:GLU43
|
3.7
|
23.5
|
1.0
|
N
|
D:GLY45
|
3.8
|
19.9
|
1.0
|
F1
|
D:ALF357
|
3.8
|
22.4
|
1.0
|
CA
|
D:GLU43
|
3.9
|
28.3
|
1.0
|
N
|
D:SER44
|
3.9
|
32.5
|
1.0
|
N
|
D:LYS46
|
3.9
|
16.3
|
1.0
|
NZ
|
D:LYS46
|
4.0
|
32.9
|
1.0
|
O
|
D:HOH365
|
4.0
|
18.9
|
1.0
|
N
|
D:SER47
|
4.0
|
24.9
|
1.0
|
O5'
|
D:GDP355
|
4.1
|
25.3
|
1.0
|
NH1
|
D:ARG178
|
4.1
|
15.3
|
1.0
|
C
|
D:GLU43
|
4.1
|
31.1
|
1.0
|
OG
|
D:SER47
|
4.3
|
24.1
|
1.0
|
C
|
D:GLY45
|
4.3
|
19.5
|
1.0
|
CA
|
D:GLY45
|
4.4
|
20.7
|
1.0
|
CB
|
D:LYS46
|
4.4
|
6.4
|
1.0
|
CE
|
D:LYS46
|
4.4
|
34.7
|
1.0
|
OG
|
D:SER44
|
4.5
|
41.2
|
1.0
|
CA
|
D:LYS46
|
4.6
|
16.5
|
1.0
|
CB
|
D:SER47
|
4.6
|
32.6
|
1.0
|
C5'
|
D:GDP355
|
4.7
|
23.2
|
1.0
|
CG
|
D:LYS46
|
4.7
|
18.4
|
1.0
|
C
|
D:SER44
|
4.8
|
20.8
|
1.0
|
CZ
|
D:ARG178
|
4.8
|
18.1
|
1.0
|
CA
|
D:SER44
|
4.8
|
25.3
|
1.0
|
C
|
D:LYS46
|
4.8
|
21.3
|
1.0
|
C
|
D:GLY42
|
4.8
|
20.2
|
1.0
|
NH2
|
D:ARG178
|
4.9
|
11.8
|
1.0
|
O
|
D:GLU43
|
4.9
|
30.1
|
1.0
|
CA
|
D:SER47
|
4.9
|
26.7
|
1.0
|
|
Phosphorus binding site 4 out
of 4 in 1agr
Go back to
Phosphorus Binding Sites List in 1agr
Phosphorus binding site 4 out
of 4 in the Complex of ALF4-Activated Gi-Alpha-1 with RGS4
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 4 of Complex of ALF4-Activated Gi-Alpha-1 with RGS4 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:P355
b:21.4
occ:1.00
|
PA
|
D:GDP355
|
0.0
|
21.4
|
1.0
|
O1A
|
D:GDP355
|
1.5
|
27.2
|
1.0
|
O2A
|
D:GDP355
|
1.5
|
30.6
|
1.0
|
O5'
|
D:GDP355
|
1.6
|
25.3
|
1.0
|
O3A
|
D:GDP355
|
1.6
|
19.4
|
1.0
|
C5'
|
D:GDP355
|
2.6
|
23.2
|
1.0
|
PB
|
D:GDP355
|
2.8
|
27.5
|
1.0
|
O2B
|
D:GDP355
|
2.9
|
17.7
|
1.0
|
O3B
|
D:GDP355
|
3.5
|
31.5
|
1.0
|
O
|
D:HOH366
|
3.5
|
14.5
|
1.0
|
OG1
|
D:THR48
|
3.7
|
39.6
|
1.0
|
NH1
|
D:ARG178
|
3.8
|
15.3
|
1.0
|
C4'
|
D:GDP355
|
3.8
|
23.4
|
1.0
|
O1B
|
D:GDP355
|
3.9
|
17.7
|
1.0
|
CA
|
D:GLY45
|
4.0
|
20.7
|
1.0
|
C3'
|
D:GDP355
|
4.0
|
17.4
|
1.0
|
N
|
D:GLY45
|
4.1
|
19.9
|
1.0
|
CB
|
D:SER47
|
4.2
|
32.6
|
1.0
|
C
|
D:GLY45
|
4.2
|
19.5
|
1.0
|
N
|
D:SER47
|
4.3
|
24.9
|
1.0
|
N
|
D:THR48
|
4.4
|
26.5
|
1.0
|
C2'
|
D:GDP355
|
4.4
|
28.1
|
1.0
|
MG
|
D:MG356
|
4.5
|
15.2
|
1.0
|
O
|
D:GLY45
|
4.6
|
17.2
|
1.0
|
CZ
|
D:ARG178
|
4.7
|
18.1
|
1.0
|
N
|
D:LYS46
|
4.7
|
16.3
|
1.0
|
CA
|
D:SER47
|
4.7
|
26.7
|
1.0
|
O4'
|
D:GDP355
|
4.7
|
33.7
|
1.0
|
CA
|
D:GLU43
|
4.7
|
28.3
|
1.0
|
OG
|
D:SER47
|
4.8
|
24.1
|
1.0
|
C
|
D:GLU43
|
4.8
|
31.1
|
1.0
|
F2
|
D:ALF357
|
4.8
|
14.2
|
1.0
|
CB
|
D:THR48
|
4.8
|
27.1
|
1.0
|
O
|
D:GLU43
|
4.9
|
30.1
|
1.0
|
C
|
D:SER47
|
4.9
|
28.4
|
1.0
|
C8
|
D:GDP355
|
5.0
|
39.6
|
1.0
|
CD
|
D:ARG178
|
5.0
|
10.0
|
1.0
|
|
Reference:
J.J.Tesmer,
D.M.Berman,
A.G.Gilman,
S.R.Sprang.
Structure of RGS4 Bound to ALF4--Activated G(I ALPHA1): Stabilization of the Transition State For Gtp Hydrolysis. Cell(Cambridge,Mass.) V. 89 251 1997.
ISSN: ISSN 0092-8674
PubMed: 9108480
DOI: 10.1016/S0092-8674(00)80204-4
Page generated: Fri Sep 25 12:53:53 2020
|