Phosphorus in PDB 1aex: Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant

All present enzymatic activity of Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant:
3.1.31.1;

The structure of Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant, PDB code: 1aex was solved by R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.10
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	48.600, 48.600, 63.400, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / n/a

The binding sites of Phosphorus atom in the Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant (pdb code 1aex). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 2 binding sites of Phosphorus where determined in the Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant, PDB code: 1aex:
Jump to Phosphorus binding site number: 1; 2;

Phosphorus binding site 1 out of 2 in the Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:P151 b:33.9 occ:1.00 P2 A:THP151 0.0 33.9 1.0 O6P A:THP151 1.5 33.2 1.0 O4P A:THP151 1.5 34.1 1.0 O5P A:THP151 1.5 29.3 1.0 O5' A:THP151 1.6 31.9 1.0 C5' A:THP151 2.6 34.9 1.0 HE A:ARG35 2.8 0.0 1.0 H2 A:HOH218 2.8 0.0 1.0 HH21 A:ARG35 2.9 0.0 1.0 HH22 A:ARG87 3.0 0.0 1.0 HH12 A:ARG87 3.2 0.0 1.0 O A:HOH218 3.5 36.8 1.0 C4' A:THP151 3.6 36.2 1.0 NE A:ARG35 3.7 26.4 1.0 NH2 A:ARG35 3.9 28.1 1.0 NH2 A:ARG87 4.0 20.2 1.0 NH1 A:ARG87 4.1 21.1 1.0 H1 A:HOH218 4.2 0.0 1.0 CZ A:ARG35 4.3 25.9 1.0 O4' A:THP151 4.3 37.9 1.0 CZ A:ARG87 4.5 23.4 1.0 CA A:CA150 4.6 67.5 1.0 HH22 A:ARG35 4.7 0.0 1.0 OD1 A:ASP40 4.7 38.5 1.0 HH21 A:ARG87 4.7 0.0 1.0 CD A:ARG35 4.8 22.9 1.0 OH A:TYR113 4.8 28.4 1.0 HH11 A:ARG87 4.9 0.0 1.0 OD2 A:ASP21 4.9 28.4 1.0 C3' A:THP151 4.9 34.6 1.0

Phosphorus binding site 2 out of 2 in the Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Staphylococcal Nuclease, Methane Thiol Disulfide to V23C Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:P151 b:42.2 occ:1.00 P1 A:THP151 0.0 42.2 1.0 O1P A:THP151 1.5 52.4 1.0 O2P A:THP151 1.5 43.6 1.0 O3P A:THP151 1.5 48.3 1.0 O3' A:THP151 1.6 44.6 1.0 C3' A:THP151 2.7 34.6 1.0 HH A:TYR85 2.8 0.0 1.0 HZ3 A:LYS84 2.8 0.0 1.0 C4' A:THP151 3.4 36.2 1.0 NZ A:LYS84 3.7 59.6 1.0 OH A:TYR85 3.7 36.6 1.0 CE A:LYS84 3.8 53.6 1.0 C2' A:THP151 3.9 28.4 1.0 HZ1 A:LYS84 4.3 0.0 1.0 O4' A:THP151 4.3 37.9 1.0 CE2 A:TYR85 4.3 38.6 1.0 HZ2 A:LYS84 4.3 0.0 1.0 C5' A:THP151 4.5 34.9 1.0 C1' A:THP151 4.5 31.6 1.0 CZ A:TYR85 4.5 34.7 1.0 HH A:TYR113 5.0 0.0 1.0

R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox. Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease. Protein Sci. V. 5 1026 1996.
ISSN: ISSN 0961-8368
PubMed: 8762134