Phosphorus in PDB 1aa6: Reduced Form of Formate Dehydrogenase H From E. Coli

All present enzymatic activity of Reduced Form of Formate Dehydrogenase H From E. Coli:
1.2.1.2;

The structure of Reduced Form of Formate Dehydrogenase H From E. Coli, PDB code: 1aa6 was solved by P.D.Sun, J.C.Boyington, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.30
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	146.400, 146.400, 82.700, 90.00, 90.00, 90.00
R / Rfree (%)	21.7 / 28.7

The binding sites of Phosphorus atom in the Reduced Form of Formate Dehydrogenase H From E. Coli (pdb code 1aa6). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 4 binding sites of Phosphorus where determined in the Reduced Form of Formate Dehydrogenase H From E. Coli, PDB code: 1aa6:
Jump to Phosphorus binding site number: 1; 2; 3; 4;

Phosphorus binding site 1 out of 4 in the Reduced Form of Formate Dehydrogenase H From E. Coli


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Reduced Form of Formate Dehydrogenase H From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:P801 b:32.2 occ:1.00 PB A:MGD801 0.0 32.2 1.0 O1B A:MGD801 1.5 33.2 1.0 O2B A:MGD801 1.5 30.4 1.0 O3B A:MGD801 1.6 34.8 1.0 O5' A:MGD801 1.6 31.8 1.0 C5' A:MGD801 2.6 26.9 1.0 PA A:MGD801 2.9 38.8 1.0 O2A A:MGD801 3.1 36.1 1.0 CB A:CYS588 3.6 44.1 1.0 O3A A:MGD801 3.7 36.6 1.0 C4' A:MGD801 3.8 24.1 1.0 O1A A:MGD801 3.9 34.8 1.0 NE2 A:GLN335 4.0 30.6 1.0 C3' A:MGD801 4.2 21.6 1.0 C10 A:MGD801 4.3 38.6 1.0 O A:HOH825 4.3 54.8 1.0 SG A:CYS588 4.4 47.2 1.0 NE A:ARG110 4.5 28.1 1.0 CG2 A:THR408 4.5 47.7 1.0 CA A:CYS588 4.6 43.3 1.0 N A:ASP404 4.6 24.7 1.0 O A:HOH854 4.6 22.5 1.0 CB A:ASP404 4.6 22.2 1.0 N A:CYS588 4.7 42.3 1.0 O A:HOH883 4.7 33.2 1.0 CG A:ARG110 5.0 21.2 1.0

Phosphorus binding site 2 out of 4 in the Reduced Form of Formate Dehydrogenase H From E. Coli


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Reduced Form of Formate Dehydrogenase H From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:P801 b:38.8 occ:1.00 PA A:MGD801 0.0 38.8 1.0 O2A A:MGD801 1.5 36.1 1.0 O1A A:MGD801 1.5 34.8 1.0 O3B A:MGD801 1.6 34.8 1.0 O3A A:MGD801 1.6 36.6 1.0 C10 A:MGD801 2.6 38.6 1.0 PB A:MGD801 2.9 32.2 1.0 O1B A:MGD801 3.1 33.2 1.0 O5' A:MGD801 3.4 31.8 1.0 O A:HOH853 3.5 40.8 1.0 C11 A:MGD801 3.7 37.8 1.0 OG1 A:THR408 3.8 48.8 1.0 O2B A:MGD801 4.0 30.4 1.0 O A:HOH883 4.1 33.2 1.0 N A:ASP404 4.2 24.7 1.0 NE2 A:GLN335 4.3 30.6 1.0 CG2 A:THR408 4.3 47.7 1.0 O11 A:MGD801 4.3 40.0 1.0 C5' A:MGD801 4.4 26.9 1.0 CB A:THR408 4.4 49.1 1.0 CA A:GLU403 4.5 23.3 1.0 O A:HOH882 4.8 35.6 1.0 CB A:GLU403 4.8 24.2 1.0 CB A:CYS136 4.8 36.3 1.0 C A:GLU403 4.9 24.3 1.0 C12 A:MGD801 4.9 38.4 1.0

Phosphorus binding site 3 out of 4 in the Reduced Form of Formate Dehydrogenase H From E. Coli


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 3 of Reduced Form of Formate Dehydrogenase H From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:P802 b:29.2 occ:1.00 PB A:MGD802 0.0 29.2 1.0 O1B A:MGD802 1.5 26.8 1.0 O2B A:MGD802 1.5 28.5 1.0 O3B A:MGD802 1.6 29.1 1.0 O5' A:MGD802 1.6 27.7 1.0 C5' A:MGD802 2.6 24.2 1.0 PA A:MGD802 3.0 32.5 1.0 NZ A:LYS679 3.2 53.0 1.0 O2A A:MGD802 3.4 30.9 1.0 O3A A:MGD802 3.4 30.2 1.0 C4' A:MGD802 3.9 23.4 1.0 CG2 A:VAL580 4.0 46.1 1.0 O1A A:MGD802 4.1 30.9 1.0 C3' A:MGD802 4.1 23.4 1.0 C10 A:MGD802 4.1 25.4 1.0 N A:ARG581 4.2 45.7 1.0 CA A:VAL580 4.2 44.3 1.0 CG A:ARG581 4.3 40.9 1.0 CE A:LYS679 4.5 53.0 1.0 O A:THR579 4.6 43.3 1.0 O4' A:MGD802 4.7 23.1 1.0 C A:VAL580 4.7 45.3 1.0 CB A:VAL580 4.8 45.2 1.0 CB A:ARG581 4.9 43.7 1.0 CB A:MET297 4.9 36.6 1.0 OG A:SER180 4.9 23.5 1.0

Phosphorus binding site 4 out of 4 in the Reduced Form of Formate Dehydrogenase H From E. Coli


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 4 of Reduced Form of Formate Dehydrogenase H From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:P802 b:32.5 occ:1.00 PA A:MGD802 0.0 32.5 1.0 O1A A:MGD802 1.5 30.9 1.0 O2A A:MGD802 1.5 30.9 1.0 O3B A:MGD802 1.6 29.1 1.0 O3A A:MGD802 1.6 30.2 1.0 C10 A:MGD802 2.7 25.4 1.0 PB A:MGD802 3.0 29.2 1.0 O1B A:MGD802 3.4 26.8 1.0 OG A:SER180 3.5 23.5 1.0 C5' A:MGD802 3.6 24.2 1.0 O5' A:MGD802 3.6 27.7 1.0 O A:HOH804 3.6 14.5 1.0 C11 A:MGD802 3.8 23.6 1.0 O2B A:MGD802 3.9 28.5 1.0 N A:MET297 4.2 31.8 1.0 O11 A:MGD802 4.3 21.6 1.0 CB A:SER180 4.3 20.0 1.0 CD1 A:TYR175 4.4 12.4 1.0 CG A:MET297 4.4 41.8 1.0 CA A:GLY296 4.6 23.5 1.0 CB A:MET297 4.7 36.6 1.0 CE1 A:TYR175 4.7 12.2 1.0 O A:GLY174 4.8 18.9 1.0 N A:ASN176 4.8 18.1 1.0 CG A:ARG581 4.9 40.9 1.0 C A:GLY296 5.0 27.3 1.0

J.C.Boyington, V.N.Gladyshev, S.V.Khangulov, T.C.Stadtman, P.D.Sun. Crystal Structure of Formate Dehydrogenase H: Catalysis Involving Mo, Molybdopterin, Selenocysteine, and An FE4S4 Cluster. Science V. 275 1305 1997.
ISSN: ISSN 0036-8075
PubMed: 9036855
DOI: 10.1126/SCIENCE.275.5304.1305