Phosphorus in PDB 1a9z: Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose

Enzymatic activity of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose

All present enzymatic activity of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose:
5.1.3.2;

Protein crystallography data

The structure of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose, PDB code: 1a9z was solved by J.B.Thoden, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.90
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 83.900, 83.900, 108.100, 90.00, 90.00, 120.00
R / Rfree (%) 18.8 / n/a

Phosphorus Binding Sites:

The binding sites of Phosphorus atom in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose (pdb code 1a9z). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 4 binding sites of Phosphorus where determined in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose, PDB code: 1a9z:
Jump to Phosphorus binding site number: 1; 2; 3; 4;

Phosphorus binding site 1 out of 4 in 1a9z

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Phosphorus binding site 1 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P340

b:21.7
occ:1.00
PA A:NAD340 0.0 21.7 1.0
O1A A:NAD340 1.5 23.7 1.0
O2A A:NAD340 1.5 27.6 1.0
O3 A:NAD340 1.5 21.9 1.0
O5B A:NAD340 1.6 20.4 1.0
C5B A:NAD340 2.6 16.3 1.0
PN A:NAD340 2.9 21.5 1.0
O2N A:NAD340 3.1 20.1 1.0
C4B A:NAD340 3.6 17.9 1.0
O5D A:NAD340 3.8 20.3 1.0
O1N A:NAD340 3.8 20.8 1.0
C3B A:NAD340 3.8 18.8 1.0
ND2 A:ASN35 3.9 20.3 1.0
CE A:LYS84 4.0 16.6 1.0
N A:TYR11 4.1 14.5 1.0
CA A:GLY10 4.1 22.0 1.0
O A:HOH447 4.2 21.8 1.0
O A:HOH503 4.2 24.8 1.0
NZ A:LYS84 4.3 23.1 1.0
C5D A:NAD340 4.5 12.1 1.0
CD A:LYS84 4.5 28.5 1.0
O A:HOH832 4.5 35.8 1.0
O A:HOH738 4.6 42.2 1.0
C A:GLY10 4.6 30.4 1.0
O3B A:NAD340 4.7 20.1 1.0
CB A:TYR11 4.8 18.4 1.0
CB A:ASN35 4.8 19.9 1.0
O4B A:NAD340 4.8 20.9 1.0
CG A:ASN35 4.9 31.3 1.0
C2B A:NAD340 5.0 19.8 1.0
CA A:TYR11 5.0 13.9 1.0

Phosphorus binding site 2 out of 4 in 1a9z

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Phosphorus binding site 2 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P340

b:21.5
occ:1.00
PN A:NAD340 0.0 21.5 1.0
O1N A:NAD340 1.5 20.8 1.0
O2N A:NAD340 1.5 20.1 1.0
O5D A:NAD340 1.6 20.3 1.0
O3 A:NAD340 1.6 21.9 1.0
C5D A:NAD340 2.6 12.1 1.0
PA A:NAD340 2.9 21.7 1.0
O5B A:NAD340 3.3 20.4 1.0
O2A A:NAD340 3.4 27.6 1.0
NZ A:LYS84 3.6 23.1 1.0
C4D A:NAD340 3.8 14.1 1.0
CD A:LYS84 3.9 28.5 1.0
CE A:LYS84 3.9 16.6 1.0
O A:HOH447 3.9 21.8 1.0
O1A A:NAD340 4.0 23.7 1.0
N7N A:NAD340 4.1 29.6 1.0
N A:ILE12 4.1 22.0 1.0
CG1 A:ILE12 4.1 20.4 1.0
CB A:TYR11 4.2 18.4 1.0
O4D A:NAD340 4.2 24.0 1.0
CD1 A:ILE12 4.3 17.8 1.0
C3D A:NAD340 4.3 23.7 1.0
C5B A:NAD340 4.3 16.3 1.0
C2N A:NAD340 4.4 16.3 1.0
C2D A:NAD340 4.4 17.1 1.0
N A:TYR11 4.5 14.5 1.0
CB A:ILE12 4.5 25.8 1.0
CA A:TYR11 4.7 13.9 1.0
CD2 A:TYR11 4.8 15.2 1.0
CA A:ILE12 4.9 19.8 1.0
C1D A:NAD340 4.9 22.2 1.0
C A:TYR11 4.9 30.3 1.0

Phosphorus binding site 3 out of 4 in 1a9z

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Phosphorus binding site 3 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 3 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P341

b:23.8
occ:1.00
PA A:UPG341 0.0 23.8 1.0
O2A A:UPG341 1.5 24.7 1.0
O1A A:UPG341 1.5 30.2 1.0
O5C A:UPG341 1.6 21.7 1.0
O3A A:UPG341 1.6 39.3 1.0
C5C A:UPG341 2.6 23.6 1.0
PB A:UPG341 2.9 26.4 1.0
O2B A:UPG341 3.3 33.3 1.0
O1B A:UPG341 3.7 31.8 1.0
NH2 A:ARG292 3.8 24.8 1.0
C4C A:UPG341 3.9 28.7 1.0
O3B A:UPG341 4.0 41.4 1.0
ND2 A:ASN179 4.0 28.4 1.0
ND2 A:ASN199 4.1 27.4 1.0
O A:HOH435 4.1 29.3 1.0
O A:HOH524 4.1 37.8 1.0
C6 A:UPG341 4.2 22.1 1.0
N A:LEU200 4.2 15.9 1.0
CA A:ASN199 4.2 25.0 1.0
O4C A:UPG341 4.3 59.3 1.0
C3C A:UPG341 4.4 19.7 1.0
CB A:ASN199 4.4 26.9 1.0
C2C A:UPG341 4.5 45.4 1.0
NH1 A:ARG292 4.7 60.8 1.0
CZ A:ARG292 4.7 50.8 1.0
CG A:ASN199 4.8 38.6 1.0
O A:ASN198 4.8 40.6 1.0
CB A:LEU200 4.8 19.9 1.0
C A:ASN199 4.8 23.4 1.0
C1C A:UPG341 4.9 32.8 1.0
C5 A:UPG341 4.9 15.8 1.0

Phosphorus binding site 4 out of 4 in 1a9z

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Phosphorus binding site 4 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 4 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P341

b:26.4
occ:1.00
PB A:UPG341 0.0 26.4 1.0
O2B A:UPG341 1.5 33.3 1.0
O1B A:UPG341 1.5 31.8 1.0
O3A A:UPG341 1.5 39.3 1.0
O3B A:UPG341 1.5 41.4 1.0
C1' A:UPG341 2.5 32.3 1.0
PA A:UPG341 2.9 23.8 1.0
ND2 A:ASN179 3.2 28.4 1.0
O5' A:UPG341 3.4 41.1 1.0
O1A A:UPG341 3.4 30.2 1.0
O5C A:UPG341 3.5 21.7 1.0
C2' A:UPG341 3.7 34.5 1.0
O A:HOH540 3.7 38.8 1.0
C5C A:UPG341 3.9 23.6 1.0
O2A A:UPG341 3.9 24.7 1.0
NE A:ARG231 4.0 24.0 1.0
O2' A:UPG341 4.0 26.4 1.0
C5' A:UPG341 4.1 35.0 1.0
CG A:ASN179 4.2 47.2 1.0
O A:HOH659 4.2 46.4 1.0
OH A:TYR299 4.2 21.5 1.0
O A:HOH524 4.4 37.8 1.0
OD1 A:ASN179 4.4 28.7 1.0
NH2 A:ARG292 4.6 24.8 1.0
CD A:ARG231 4.6 24.9 1.0
C3' A:UPG341 4.7 25.6 1.0
O A:HOH435 4.8 29.3 1.0
CZ A:ARG231 5.0 20.8 1.0
NH2 A:ARG231 5.0 30.6 1.0

Reference:

J.B.Thoden, H.M.Holden. Dramatic Differences in the Binding of Udp-Galactose and Udp-Glucose to Udp-Galactose 4-Epimerase From Escherichia Coli. Biochemistry V. 37 11469 1998.
ISSN: ISSN 0006-2960
PubMed: 9708982
DOI: 10.1021/BI9808969
Page generated: Fri Sep 25 12:39:37 2020

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