Phosphorus in PDB 1a9y: Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose

All present enzymatic activity of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose:
5.1.3.2;

The structure of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose, PDB code: 1a9y was solved by J.B.Thoden, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	85.000, 85.000, 106.700, 90.00, 90.00, 120.00
R / Rfree (%)	18.1 / n/a

The binding sites of Phosphorus atom in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose (pdb code 1a9y). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 4 binding sites of Phosphorus where determined in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose, PDB code: 1a9y:
Jump to Phosphorus binding site number: 1; 2; 3; 4;

Phosphorus binding site 1 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose within 5.0Å range: probe atom residue distance (Å) B Occ A:P340 b:16.5 occ:1.00 PA A:NAD340 0.0 16.5 1.0 O1A A:NAD340 1.5 16.2 1.0 O2A A:NAD340 1.5 12.5 1.0 O3 A:NAD340 1.6 16.3 1.0 O5B A:NAD340 1.6 16.4 1.0 C5B A:NAD340 2.7 10.9 1.0 PN A:NAD340 2.9 18.1 1.0 O2N A:NAD340 3.1 19.1 1.0 C4B A:NAD340 3.7 14.8 1.0 C3B A:NAD340 3.7 13.7 1.0 O1N A:NAD340 3.8 16.2 1.0 O5D A:NAD340 3.9 16.7 1.0 N A:TYR11 3.9 19.6 1.0 CA A:GLY10 4.1 15.4 1.0 O A:HOH607 4.2 31.4 1.0 ND2 A:ASN35 4.2 27.1 1.0 O A:HOH668 4.2 42.3 1.0 O A:HOH422 4.2 17.0 1.0 O A:HOH499 4.2 18.8 1.0 CE A:LYS84 4.2 18.7 1.0 NZ A:LYS84 4.4 23.8 1.0 CD A:LYS84 4.4 21.3 1.0 C5D A:NAD340 4.5 13.6 1.0 O3B A:NAD340 4.5 16.3 1.0 C A:GLY10 4.5 26.4 1.0 CB A:TYR11 4.7 14.8 1.0 O4B A:NAD340 4.9 12.8 1.0 CA A:TYR11 4.9 17.0 1.0 C2B A:NAD340 4.9 24.8 1.0

Phosphorus binding site 2 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose within 5.0Å range: probe atom residue distance (Å) B Occ A:P340 b:18.1 occ:1.00 PN A:NAD340 0.0 18.1 1.0 O2N A:NAD340 1.5 19.1 1.0 O1N A:NAD340 1.5 16.2 1.0 O5D A:NAD340 1.6 16.7 1.0 O3 A:NAD340 1.6 16.3 1.0 C5D A:NAD340 2.7 13.6 1.0 PA A:NAD340 2.9 16.5 1.0 O2A A:NAD340 3.3 12.5 1.0 O5B A:NAD340 3.3 16.4 1.0 NZ A:LYS84 3.7 23.8 1.0 O A:HOH422 3.9 17.0 1.0 C4D A:NAD340 3.9 12.9 1.0 CD A:LYS84 3.9 21.3 1.0 CG1 A:ILE12 4.0 20.4 1.0 N A:ILE12 4.0 17.4 1.0 CB A:TYR11 4.0 14.8 1.0 CD1 A:ILE12 4.1 15.5 1.0 O1A A:NAD340 4.1 16.2 1.0 N7N A:NAD340 4.1 13.8 1.0 CE A:LYS84 4.2 18.7 1.0 O4D A:NAD340 4.2 16.1 1.0 C5B A:NAD340 4.3 10.9 1.0 N A:TYR11 4.4 19.6 1.0 C3D A:NAD340 4.4 20.2 1.0 CB A:ILE12 4.4 19.2 1.0 C2D A:NAD340 4.4 20.9 1.0 C2N A:NAD340 4.5 14.7 1.0 CA A:TYR11 4.6 17.0 1.0 CD2 A:TYR11 4.6 18.5 1.0 C A:TYR11 4.8 13.9 1.0 CG A:TYR11 4.8 11.6 1.0 CA A:ILE12 4.9 16.3 1.0 C1D A:NAD340 4.9 14.1 1.0

Phosphorus binding site 3 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 3 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose within 5.0Å range: probe atom residue distance (Å) B Occ A:P341 b:16.1 occ:1.00 PA A:UPG341 0.0 16.1 1.0 O1A A:UPG341 1.5 21.4 1.0 O2A A:UPG341 1.5 21.0 1.0 O5C A:UPG341 1.6 17.0 1.0 O3A A:UPG341 1.7 25.4 1.0 C5C A:UPG341 2.6 13.4 1.0 PB A:UPG341 2.9 22.8 1.0 O2B A:UPG341 3.3 25.1 1.0 NH2 A:ARG292 3.5 21.9 1.0 ND2 A:ASN199 3.7 29.4 1.0 C4C A:UPG341 3.8 16.9 1.0 O A:HOH440 3.8 22.7 1.0 O3B A:UPG341 3.8 32.9 1.0 O1B A:UPG341 3.9 15.1 1.0 C6 A:UPG341 3.9 10.2 1.0 N A:LEU200 3.9 14.8 1.0 CA A:ASN199 4.0 21.0 1.0 O4C A:UPG341 4.0 22.4 1.0 C2C A:UPG341 4.3 17.5 1.0 C3C A:UPG341 4.3 10.7 1.0 NH1 A:ARG292 4.3 21.2 1.0 ND2 A:ASN179 4.3 21.5 1.0 O A:HOH502 4.4 34.0 1.0 O A:ASN198 4.4 26.9 1.0 CZ A:ARG292 4.4 18.8 1.0 CB A:ASN199 4.4 17.4 1.0 C1' A:UPG341 4.5 31.5 1.0 C A:ASN199 4.5 18.1 1.0 CG A:ASN199 4.5 27.6 1.0 CB A:LEU200 4.6 12.1 1.0 C5 A:UPG341 4.6 11.4 1.0 C1C A:UPG341 4.7 14.8 1.0 N1 A:UPG341 4.8 13.6 1.0 CA A:LEU200 4.9 17.6 1.0

Phosphorus binding site 4 out of 4 in the Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 4 of Udp-Galactose 4-Epimerase Mutant S124A/Y149F Complexed with Udp- Glucose within 5.0Å range: probe atom residue distance (Å) B Occ A:P341 b:22.8 occ:1.00 PB A:UPG341 0.0 22.8 1.0 O2B A:UPG341 1.5 25.1 1.0 O1B A:UPG341 1.5 15.1 1.0 O3A A:UPG341 1.5 25.4 1.0 O3B A:UPG341 1.6 32.9 1.0 C1' A:UPG341 2.7 31.5 1.0 PA A:UPG341 2.9 16.1 1.0 O5' A:UPG341 3.3 28.3 1.0 O5C A:UPG341 3.4 17.0 1.0 O1A A:UPG341 3.4 21.4 1.0 ND2 A:ASN179 3.5 21.5 1.0 O A:HOH429 3.6 21.6 1.0 O A:HOH524 3.6 32.3 1.0 C5C A:UPG341 3.9 13.4 1.0 C2' A:UPG341 3.9 37.2 1.0 O2A A:UPG341 4.0 21.0 1.0 NH2 A:ARG292 4.0 21.9 1.0 O A:HOH502 4.0 34.0 1.0 C5' A:UPG341 4.0 27.0 1.0 NE A:ARG231 4.2 10.5 1.0 O2' A:UPG341 4.2 24.4 1.0 O A:HOH855 4.5 38.5 1.0 C3' A:UPG341 4.5 19.7 1.0 OH A:TYR299 4.6 16.7 1.0 CG A:ASN179 4.7 20.6 1.0 ND2 A:ASN199 4.7 29.4 1.0 CD A:ARG231 4.8 11.3 1.0 O A:HOH440 4.8 22.7 1.0 O6' A:UPG341 4.9 20.0 1.0 C3C A:UPG341 4.9 10.7 1.0 C4' A:UPG341 5.0 31.5 1.0 C4C A:UPG341 5.0 16.9 1.0

J.B.Thoden, H.M.Holden. Dramatic Differences in the Binding of Udp-Galactose and Udp-Glucose to Udp-Galactose 4-Epimerase From Escherichia Coli. Biochemistry V. 37 11469 1998.
ISSN: ISSN 0006-2960
PubMed: 9708982
DOI: 10.1021/BI9808969