Phosphorus in PDB 1a5a: Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49

Enzymatic activity of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49

All present enzymatic activity of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49:
4.2.1.20;

Protein crystallography data

The structure of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49, PDB code: 1a5a was solved by S.Rhee, E.W.Miles, D.R.Davies, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 1.90
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 183.500, 59.400, 67.300, 90.00, 94.60, 90.00
R / Rfree (%) 23.8 / 29.8

Phosphorus Binding Sites:

The binding sites of Phosphorus atom in the Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49 (pdb code 1a5a). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total only one binding site of Phosphorus was determined in the Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49, PDB code: 1a5a:

Phosphorus binding site 1 out of 1 in 1a5a

Go back to Phosphorus Binding Sites List in 1a5a
Phosphorus binding site 1 out of 1 in the Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:P501

b:13.7
occ:1.00
 P B:PLP501 0.0 13.7 1.0
O3P B:PLP501 1.4 10.4 1.0
O1P B:PLP501 1.5 15.9 1.0
O4P B:PLP501 1.5 14.6 1.0
O2P B:PLP501 1.5 18.0 1.0
C5A B:PLP501 2.7 18.0 1.0
C5 B:PLP501 3.4 17.7 1.0
OG B:SER235 3.5 9.8 1.0
O B:HOH833 3.6 19.5 1.0
N B:SER235 3.7 9.0 1.0
N B:GLY233 3.7 14.1 1.0
N B:GLY234 3.7 6.8 1.0
O B:HOH598 3.8 22.4 1.0
ND2 B:ASN236 4.0 2.0 1.0
N B:GLY232 4.0 10.9 1.0
O B:HOH834 4.0 31.3 1.0
C6 B:PLP501 4.0 15.9 1.0
N B:ASN236 4.1 6.6 1.0
OG1 B:THR190 4.2 14.2 1.0
NE2 B:HIS86 4.2 3.6 1.0
CA B:GLY232 4.3 9.9 1.0
C B:GLY234 4.4 9.2 1.0
CA B:GLY234 4.4 3.4 1.0
C4 B:PLP501 4.4 20.9 1.0
CA B:SER235 4.4 7.7 1.0
CE1 B:HIS86 4.5 3.3 1.0
CA B:GLY233 4.5 9.4 1.0
C B:GLY232 4.5 16.4 1.0
C4A B:PLP501 4.5 23.0 1.0
CB B:SER235 4.5 6.5 1.0
C B:GLY233 4.6 7.8 1.0
C B:SER235 4.8 5.6 1.0
CG B:ASN236 4.9 4.1 1.0
CB B:ASN236 4.9 8.6 1.0
CB B:THR190 4.9 13.1 1.0
O B:HOH769 4.9 34.7 1.0

Reference:

S.Rhee, E.W.Miles, D.R.Davies. Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site ALPHAGLU49. J.Biol.Chem. V. 273 8553 1998.
ISSN: ISSN 0021-9258
PubMed: 9535826
DOI: 10.1074/JBC.273.15.8553
Page generated: Fri Sep 25 12:16:43 2020

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