Phosphorus in PDB 1a2t: Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant

Enzymatic activity of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant

All present enzymatic activity of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant:
3.1.31.1;

Protein crystallography data

The structure of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant, PDB code: 1a2t was solved by R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 6.00 / 1.96
Space group P 41
Cell size a, b, c (Å), α, β, γ (°) 48.200, 48.200, 63.000, 90.00, 90.00, 90.00
R / Rfree (%) 16.9 / n/a

Phosphorus Binding Sites:

The binding sites of Phosphorus atom in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant (pdb code 1a2t). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 2 binding sites of Phosphorus where determined in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant, PDB code: 1a2t:
Jump to Phosphorus binding site number: 1; 2;

Phosphorus binding site 1 out of 2 in 1a2t

Go back to Phosphorus Binding Sites List in 1a2t
Phosphorus binding site 1 out of 2 in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P151

b:36.0
occ:1.00
P2 A:THP151 0.0 36.0 1.0
O6P A:THP151 1.5 37.0 1.0
O5P A:THP151 1.5 33.2 1.0
O4P A:THP151 1.5 36.4 1.0
O5' A:THP151 1.6 36.0 1.0
HE A:ARG35 2.7 0.0 1.0
C5' A:THP151 2.7 36.4 1.0
HH21 A:ARG35 2.8 0.0 1.0
H1 A:HOH215 2.9 0.0 1.0
HH22 A:ARG87 3.0 0.0 1.0
HH12 A:ARG87 3.2 0.0 1.0
H1 A:HOH214 3.4 0.0 1.0
H2 A:HOH229 3.5 0.0 1.0
O A:HOH215 3.6 30.6 1.0
NE A:ARG35 3.6 27.1 1.0
C4' A:THP151 3.7 35.6 1.0
CA A:CA150 3.7 34.2 1.0
NH2 A:ARG35 3.7 32.8 1.0
O A:HOH214 3.9 55.2 1.0
NH2 A:ARG87 4.0 24.8 1.0
NH1 A:ARG87 4.1 24.6 1.0
CZ A:ARG35 4.2 30.2 1.0
O4' A:THP151 4.3 33.8 1.0
H2 A:HOH215 4.3 0.0 1.0
OD1 A:ASP40 4.4 38.4 1.0
O A:HOH229 4.5 54.8 1.0
CZ A:ARG87 4.5 27.4 1.0
HH22 A:ARG35 4.6 0.0 1.0
HH21 A:ARG87 4.7 0.0 1.0
CD A:ARG35 4.7 25.6 1.0
H2 A:HOH214 4.7 0.0 1.0
OH A:TYR113 4.8 34.4 1.0
H1 A:HOH229 4.9 0.0 1.0
OD2 A:ASP21 4.9 27.4 1.0
OE1 A:GLU43 4.9 46.2 1.0
HH11 A:ARG87 4.9 0.0 1.0
C3' A:THP151 5.0 35.7 1.0

Phosphorus binding site 2 out of 2 in 1a2t

Go back to Phosphorus Binding Sites List in 1a2t
Phosphorus binding site 2 out of 2 in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P151

b:38.0
occ:1.00
P1 A:THP151 0.0 38.0 1.0
O3P A:THP151 1.5 46.1 1.0
O2P A:THP151 1.5 39.0 1.0
O1P A:THP151 1.5 46.2 1.0
O3' A:THP151 1.6 40.6 1.0
HH A:TYR85 2.5 0.0 1.0
C3' A:THP151 2.7 35.7 1.0
HZ3 A:LYS84 2.7 0.0 1.0
H1 A:HOH229 3.1 0.0 1.0
C4' A:THP151 3.4 35.6 1.0
OH A:TYR85 3.5 39.4 1.0
NZ A:LYS84 3.7 49.4 1.0
C2' A:THP151 4.0 31.3 1.0
HZ2 A:LYS84 4.0 0.0 1.0
O A:HOH229 4.0 54.8 1.0
CE A:LYS84 4.1 41.8 1.0
H2 A:HOH229 4.1 0.0 1.0
CE2 A:TYR85 4.2 33.2 1.0
CZ A:TYR85 4.3 36.5 1.0
O4' A:THP151 4.3 33.8 1.0
HZ1 A:LYS84 4.4 0.0 1.0
C5' A:THP151 4.5 36.4 1.0
C1' A:THP151 4.5 31.6 1.0

Reference:

R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox. Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease. Protein Sci. V. 5 1026 1996.
ISSN: ISSN 0961-8368
PubMed: 8762134
Page generated: Fri Sep 25 12:04:17 2020

Last articles

Na in 5RCN
Na in 5RCK
Na in 5RCJ
Na in 5RCI
Na in 5RCD
Na in 5RC2
Na in 5RCA
Na in 5RCB
Na in 5RC8
Na in 5RC5
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy