Phosphorus in PDB 1a2t: Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant

All present enzymatic activity of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant:
3.1.31.1;

The structure of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant, PDB code: 1a2t was solved by R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 1.96
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	48.200, 48.200, 63.000, 90.00, 90.00, 90.00
R / Rfree (%)	16.9 / n/a

The binding sites of Phosphorus atom in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant (pdb code 1a2t). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 2 binding sites of Phosphorus where determined in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant, PDB code: 1a2t:
Jump to Phosphorus binding site number: 1; 2;

Phosphorus binding site 1 out of 2 in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:P151 b:36.0 occ:1.00 P2 A:THP151 0.0 36.0 1.0 O6P A:THP151 1.5 37.0 1.0 O5P A:THP151 1.5 33.2 1.0 O4P A:THP151 1.5 36.4 1.0 O5' A:THP151 1.6 36.0 1.0 HE A:ARG35 2.7 0.0 1.0 C5' A:THP151 2.7 36.4 1.0 HH21 A:ARG35 2.8 0.0 1.0 H1 A:HOH215 2.9 0.0 1.0 HH22 A:ARG87 3.0 0.0 1.0 HH12 A:ARG87 3.2 0.0 1.0 H1 A:HOH214 3.4 0.0 1.0 H2 A:HOH229 3.5 0.0 1.0 O A:HOH215 3.6 30.6 1.0 NE A:ARG35 3.6 27.1 1.0 C4' A:THP151 3.7 35.6 1.0 CA A:CA150 3.7 34.2 1.0 NH2 A:ARG35 3.7 32.8 1.0 O A:HOH214 3.9 55.2 1.0 NH2 A:ARG87 4.0 24.8 1.0 NH1 A:ARG87 4.1 24.6 1.0 CZ A:ARG35 4.2 30.2 1.0 O4' A:THP151 4.3 33.8 1.0 H2 A:HOH215 4.3 0.0 1.0 OD1 A:ASP40 4.4 38.4 1.0 O A:HOH229 4.5 54.8 1.0 CZ A:ARG87 4.5 27.4 1.0 HH22 A:ARG35 4.6 0.0 1.0 HH21 A:ARG87 4.7 0.0 1.0 CD A:ARG35 4.7 25.6 1.0 H2 A:HOH214 4.7 0.0 1.0 OH A:TYR113 4.8 34.4 1.0 H1 A:HOH229 4.9 0.0 1.0 OD2 A:ASP21 4.9 27.4 1.0 OE1 A:GLU43 4.9 46.2 1.0 HH11 A:ARG87 4.9 0.0 1.0 C3' A:THP151 5.0 35.7 1.0

Phosphorus binding site 2 out of 2 in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:P151 b:38.0 occ:1.00 P1 A:THP151 0.0 38.0 1.0 O3P A:THP151 1.5 46.1 1.0 O2P A:THP151 1.5 39.0 1.0 O1P A:THP151 1.5 46.2 1.0 O3' A:THP151 1.6 40.6 1.0 HH A:TYR85 2.5 0.0 1.0 C3' A:THP151 2.7 35.7 1.0 HZ3 A:LYS84 2.7 0.0 1.0 H1 A:HOH229 3.1 0.0 1.0 C4' A:THP151 3.4 35.6 1.0 OH A:TYR85 3.5 39.4 1.0 NZ A:LYS84 3.7 49.4 1.0 C2' A:THP151 4.0 31.3 1.0 HZ2 A:LYS84 4.0 0.0 1.0 O A:HOH229 4.0 54.8 1.0 CE A:LYS84 4.1 41.8 1.0 H2 A:HOH229 4.1 0.0 1.0 CE2 A:TYR85 4.2 33.2 1.0 CZ A:TYR85 4.3 36.5 1.0 O4' A:THP151 4.3 33.8 1.0 HZ1 A:LYS84 4.4 0.0 1.0 C5' A:THP151 4.5 36.4 1.0 C1' A:THP151 4.5 31.6 1.0

R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox. Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease. Protein Sci. V. 5 1026 1996.
ISSN: ISSN 0961-8368
PubMed: 8762134