Phosphorus in PDB 1a2t: Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant

Enzymatic activity of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant

All present enzymatic activity of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant:
3.1.31.1;

Protein crystallography data

The structure of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant, PDB code: 1a2t was solved by R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 6.00 / 1.96
Space group P 41
Cell size a, b, c (Å), α, β, γ (°) 48.200, 48.200, 63.000, 90.00, 90.00, 90.00
R / Rfree (%) 16.9 / n/a

Phosphorus Binding Sites:

The binding sites of Phosphorus atom in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant (pdb code 1a2t). This binding sites where shown within 5.0 Angstroms radius around Phosphorus atom.
In total 2 binding sites of Phosphorus where determined in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant, PDB code: 1a2t:
Jump to Phosphorus binding site number: 1; 2;

Phosphorus binding site 1 out of 2 in 1a2t

Go back to Phosphorus Binding Sites List in 1a2t
Phosphorus binding site 1 out of 2 in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 1 of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P151

b:36.0
occ:1.00
P2 A:THP151 0.0 36.0 1.0
O6P A:THP151 1.5 37.0 1.0
O5P A:THP151 1.5 33.2 1.0
O4P A:THP151 1.5 36.4 1.0
O5' A:THP151 1.6 36.0 1.0
HE A:ARG35 2.7 0.0 1.0
C5' A:THP151 2.7 36.4 1.0
HH21 A:ARG35 2.8 0.0 1.0
H1 A:HOH215 2.9 0.0 1.0
HH22 A:ARG87 3.0 0.0 1.0
HH12 A:ARG87 3.2 0.0 1.0
H1 A:HOH214 3.4 0.0 1.0
H2 A:HOH229 3.5 0.0 1.0
O A:HOH215 3.6 30.6 1.0
NE A:ARG35 3.6 27.1 1.0
C4' A:THP151 3.7 35.6 1.0
CA A:CA150 3.7 34.2 1.0
NH2 A:ARG35 3.7 32.8 1.0
O A:HOH214 3.9 55.2 1.0
NH2 A:ARG87 4.0 24.8 1.0
NH1 A:ARG87 4.1 24.6 1.0
CZ A:ARG35 4.2 30.2 1.0
O4' A:THP151 4.3 33.8 1.0
H2 A:HOH215 4.3 0.0 1.0
OD1 A:ASP40 4.4 38.4 1.0
O A:HOH229 4.5 54.8 1.0
CZ A:ARG87 4.5 27.4 1.0
HH22 A:ARG35 4.6 0.0 1.0
HH21 A:ARG87 4.7 0.0 1.0
CD A:ARG35 4.7 25.6 1.0
H2 A:HOH214 4.7 0.0 1.0
OH A:TYR113 4.8 34.4 1.0
H1 A:HOH229 4.9 0.0 1.0
OD2 A:ASP21 4.9 27.4 1.0
OE1 A:GLU43 4.9 46.2 1.0
HH11 A:ARG87 4.9 0.0 1.0
C3' A:THP151 5.0 35.7 1.0

Phosphorus binding site 2 out of 2 in 1a2t

Go back to Phosphorus Binding Sites List in 1a2t
Phosphorus binding site 2 out of 2 in the Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant


Mono view


Stereo pair view

A full contact list of Phosphorus with other atoms in the P binding site number 2 of Staphylococcal Nuclease, B-Mercaptoethanol Disulfide to V23C Variant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:P151

b:38.0
occ:1.00
P1 A:THP151 0.0 38.0 1.0
O3P A:THP151 1.5 46.1 1.0
O2P A:THP151 1.5 39.0 1.0
O1P A:THP151 1.5 46.2 1.0
O3' A:THP151 1.6 40.6 1.0
HH A:TYR85 2.5 0.0 1.0
C3' A:THP151 2.7 35.7 1.0
HZ3 A:LYS84 2.7 0.0 1.0
H1 A:HOH229 3.1 0.0 1.0
C4' A:THP151 3.4 35.6 1.0
OH A:TYR85 3.5 39.4 1.0
NZ A:LYS84 3.7 49.4 1.0
C2' A:THP151 4.0 31.3 1.0
HZ2 A:LYS84 4.0 0.0 1.0
O A:HOH229 4.0 54.8 1.0
CE A:LYS84 4.1 41.8 1.0
H2 A:HOH229 4.1 0.0 1.0
CE2 A:TYR85 4.2 33.2 1.0
CZ A:TYR85 4.3 36.5 1.0
O4' A:THP151 4.3 33.8 1.0
HZ1 A:LYS84 4.4 0.0 1.0
C5' A:THP151 4.5 36.4 1.0
C1' A:THP151 4.5 31.6 1.0

Reference:

R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox. Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease. Protein Sci. V. 5 1026 1996.
ISSN: ISSN 0961-8368
PubMed: 8762134
Page generated: Fri Sep 25 12:04:17 2020

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