Phosphorus in PDB 1a27: Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+
Enzymatic activity of Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+
All present enzymatic activity of Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+:
1.1.1.62;
Protein crystallography data
The structure of Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+, PDB code: 1a27
was solved by
C.Mazza,
R.Breton,
D.Housset,
J.-C.Fontecilla-Camps,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
10.00 /
1.90
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
111.380,
48.540,
60.770,
90.00,
115.13,
90.00
|
R / Rfree (%)
|
21 /
26.1
|
Phosphorus Binding Sites:
The binding sites of Phosphorus atom in the Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+
(pdb code 1a27). This binding sites where shown within
5.0 Angstroms radius around Phosphorus atom.
In total 3 binding sites of Phosphorus where determined in the
Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+, PDB code: 1a27:
Jump to Phosphorus binding site number:
1;
2;
3;
Phosphorus binding site 1 out
of 3 in 1a27
Go back to
Phosphorus Binding Sites List in 1a27
Phosphorus binding site 1 out
of 3 in the Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 1 of Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+ within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:P360
b:39.8
occ:1.00
|
PA
|
A:NAP360
|
0.0
|
39.8
|
1.0
|
O1A
|
A:NAP360
|
1.5
|
38.3
|
1.0
|
O2A
|
A:NAP360
|
1.5
|
38.5
|
1.0
|
O3
|
A:NAP360
|
1.6
|
37.4
|
1.0
|
O5B
|
A:NAP360
|
1.6
|
39.7
|
1.0
|
C5B
|
A:NAP360
|
2.7
|
38.6
|
1.0
|
PN
|
A:NAP360
|
2.9
|
38.8
|
1.0
|
O2N
|
A:NAP360
|
3.2
|
37.1
|
1.0
|
OG
|
A:SER12
|
3.5
|
27.4
|
1.0
|
O
|
A:HOH603
|
3.6
|
50.4
|
1.0
|
O5D
|
A:NAP360
|
3.6
|
37.5
|
1.0
|
O
|
A:THR190
|
3.8
|
53.4
|
1.0
|
C4B
|
A:NAP360
|
3.9
|
37.1
|
1.0
|
O
|
A:HOH573
|
3.9
|
49.7
|
1.0
|
O1N
|
A:NAP360
|
4.1
|
39.4
|
1.0
|
C3B
|
A:NAP360
|
4.1
|
37.3
|
1.0
|
CA
|
A:PHE192
|
4.2
|
71.5
|
0.5
|
CB
|
A:PHE192
|
4.2
|
72.2
|
0.5
|
OG1
|
A:THR190
|
4.3
|
46.0
|
1.0
|
CB
|
A:THR190
|
4.3
|
47.7
|
1.0
|
N
|
A:PHE192
|
4.3
|
68.3
|
0.5
|
O
|
A:HOH525
|
4.4
|
28.7
|
1.0
|
CD1
|
A:PHE192
|
4.5
|
73.0
|
0.5
|
C
|
A:THR190
|
4.7
|
52.0
|
1.0
|
O
|
A:HOH605
|
4.7
|
46.0
|
1.0
|
O
|
A:HOH618
|
4.7
|
77.8
|
1.0
|
CB
|
A:SER12
|
4.8
|
26.4
|
1.0
|
C
|
A:ALA191
|
4.8
|
73.0
|
0.5
|
C5D
|
A:NAP360
|
4.8
|
37.6
|
1.0
|
C
|
A:SER12
|
4.8
|
25.0
|
1.0
|
O
|
A:SER12
|
4.8
|
23.7
|
1.0
|
CG
|
A:PHE192
|
4.8
|
72.8
|
0.5
|
N
|
A:GLY13
|
4.8
|
25.6
|
1.0
|
CA
|
A:GLY13
|
4.9
|
23.7
|
1.0
|
O4B
|
A:NAP360
|
5.0
|
37.5
|
1.0
|
|
Phosphorus binding site 2 out
of 3 in 1a27
Go back to
Phosphorus Binding Sites List in 1a27
Phosphorus binding site 2 out
of 3 in the Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 2 of Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+ within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:P360
b:38.8
occ:1.00
|
PN
|
A:NAP360
|
0.0
|
38.8
|
1.0
|
O2N
|
A:NAP360
|
1.5
|
37.1
|
1.0
|
O1N
|
A:NAP360
|
1.5
|
39.4
|
1.0
|
O5D
|
A:NAP360
|
1.6
|
37.5
|
1.0
|
O3
|
A:NAP360
|
1.6
|
37.4
|
1.0
|
C5D
|
A:NAP360
|
2.7
|
37.6
|
1.0
|
O
|
A:HOH618
|
2.9
|
77.8
|
1.0
|
PA
|
A:NAP360
|
2.9
|
39.8
|
1.0
|
O5B
|
A:NAP360
|
3.2
|
39.7
|
1.0
|
O2A
|
A:NAP360
|
3.4
|
38.5
|
1.0
|
OG1
|
A:THR190
|
3.4
|
46.0
|
1.0
|
C5B
|
A:NAP360
|
3.8
|
38.6
|
1.0
|
C4D
|
A:NAP360
|
3.8
|
39.1
|
1.0
|
O4D
|
A:NAP360
|
3.8
|
38.3
|
1.0
|
CB
|
A:THR190
|
3.8
|
47.7
|
1.0
|
O
|
A:HOH525
|
3.8
|
28.7
|
1.0
|
CG2
|
A:THR190
|
3.9
|
46.8
|
1.0
|
CB
|
A:PHE192
|
4.0
|
72.2
|
0.5
|
N7N
|
A:NAP360
|
4.1
|
42.2
|
1.0
|
O1A
|
A:NAP360
|
4.1
|
38.3
|
1.0
|
CG
|
A:PHE192
|
4.3
|
72.8
|
0.5
|
C2N
|
A:NAP360
|
4.3
|
39.5
|
1.0
|
CD1
|
A:PHE192
|
4.4
|
73.0
|
0.5
|
C3D
|
A:NAP360
|
4.5
|
38.3
|
1.0
|
N
|
A:ILE14
|
4.5
|
22.8
|
1.0
|
CG1
|
A:ILE14
|
4.5
|
27.9
|
1.0
|
CA
|
A:GLY13
|
4.7
|
23.7
|
1.0
|
C1D
|
A:NAP360
|
4.7
|
38.8
|
1.0
|
C2D
|
A:NAP360
|
4.8
|
39.2
|
1.0
|
CD1
|
A:ILE14
|
4.8
|
29.8
|
1.0
|
CA
|
A:PHE192
|
4.9
|
71.5
|
0.5
|
|
Phosphorus binding site 3 out
of 3 in 1a27
Go back to
Phosphorus Binding Sites List in 1a27
Phosphorus binding site 3 out
of 3 in the Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+
Mono view
Stereo pair view
|
A full contact list of Phosphorus with other atoms in the P binding
site number 3 of Human 17-Beta-Hydroxysteroid-Dehydrogenase Type 1 C-Terminal Deletion Mutant Complexed with Estradiol and Nadp+ within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:P360
b:46.9
occ:1.00
|
P2B
|
A:NAP360
|
0.0
|
46.9
|
1.0
|
O1X
|
A:NAP360
|
1.4
|
47.6
|
1.0
|
O2B
|
A:NAP360
|
1.4
|
42.5
|
1.0
|
O2X
|
A:NAP360
|
1.4
|
45.8
|
1.0
|
O3X
|
A:NAP360
|
1.4
|
47.2
|
1.0
|
C2B
|
A:NAP360
|
2.5
|
38.6
|
1.0
|
C3B
|
A:NAP360
|
3.5
|
37.3
|
1.0
|
O
|
A:HOH550
|
3.6
|
21.9
|
1.0
|
C1B
|
A:NAP360
|
3.7
|
37.2
|
1.0
|
OG
|
A:SER11
|
3.7
|
28.0
|
1.0
|
O3B
|
A:NAP360
|
3.8
|
34.2
|
1.0
|
CZ
|
A:ARG37
|
3.8
|
52.0
|
1.0
|
O
|
A:HOH605
|
3.8
|
46.0
|
1.0
|
O
|
A:HOH524
|
3.8
|
18.8
|
1.0
|
NH1
|
A:ARG37
|
3.9
|
54.0
|
1.0
|
NH2
|
A:ARG37
|
3.9
|
50.5
|
1.0
|
N9A
|
A:NAP360
|
4.0
|
36.3
|
1.0
|
C8A
|
A:NAP360
|
4.2
|
34.6
|
1.0
|
NE
|
A:ARG37
|
4.2
|
48.6
|
1.0
|
O
|
A:HOH603
|
4.3
|
50.4
|
1.0
|
NZ
|
A:LYS195
|
4.5
|
87.3
|
0.5
|
CB
|
A:ARG37
|
4.7
|
30.5
|
1.0
|
CB
|
A:SER11
|
4.7
|
22.8
|
1.0
|
C4A
|
A:NAP360
|
4.7
|
35.6
|
1.0
|
C4B
|
A:NAP360
|
4.8
|
37.1
|
1.0
|
O4B
|
A:NAP360
|
4.8
|
37.5
|
1.0
|
CD
|
A:ARG37
|
4.8
|
41.6
|
1.0
|
N7A
|
A:NAP360
|
4.9
|
34.2
|
1.0
|
|
Reference:
C.Mazza,
C.Mazza,
R.Breton,
D.Housset,
J.-C.Fontecilla-Camps.
N/A N/A.
Page generated: Fri Sep 25 12:02:54 2020
|